Phosphate buffer pH 7.2

Phosphate buffered (PB) is a balanced salt solution and is one of the most commonly used buffers for adsorptive immobilization of proteins and antibodies on plastic surfaces for ELISA, EIA, RIA, proteins array and immuno-PCR applications.

It’s a buffer isotonic and non-toxic to cells and has the ability to maintain osmolarity and for this is used for cells culture applications (for example washing cells, transporting cells or tissue samples, diluting cells for counting and preparing reagents). It is also often used for sample dilution in molecular biology and as protein diluent in Western blotting.